| FLIM - Fluorescence Lifetime Imaging Microscopy | |||
| Whole-field imaging system based on Intensified CCD Technology LaVision BioTec offers the first FLIM system with 3D spatial and temporal resolution. It is based on the intensified PicoStar CCD camera and the advanced ImSpector software package. The system can be used for time domain (down to 50 ps) as well as for frequency domain (up to 800 MHz) measurements. The FLIM system can be combined with different kinds of laser sources, microscopes, spectrographs and LaVision BioTec's outstanding TriM Scope. |
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| Applications FLIM can be used for various applications, for example  FRET (Förster Resonance Energy Transfer FRAP (Fluorescence Recovery After Photo-Bleaching Pump-Probe Spectroscopy Multi-Labelling Experiments Excited State Relaxation measurements |
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 FLIM and the combination with other techniques delivers the functional data concerning: Ca2+ concentration Oxygen concentation pH Kinetics of diffusion and absorption Ligand-receptor interaction Molecular structure and dynamics Revelation of bonding conditions and molecule environment interactions |
Setup A pulsed laser excites fluorophores in the sample. An optical trigger in combination with a delay generator opens the gate of the intensified PicoStar CCD camera which measures fluorescence signal in dependance of the delay and gate time. |
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| Gate Shift Time Domain The acquisition of images at successive delays generates a set of images with different intensity allocations which depend n the lifetime of the fluorophores. The set of data will be processed by LaVision BioTec's ImSpector software package. Advanced software algorithms compute single as well as multi-exponential lifetime images in real time. The system also provides the functionality to work frequency domain to capture fluorescence lifetime images. |
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2D/3D Capabilities 2D FLIM systems work with various fs puls laserrs and wide field illumination of the sample. Whereas sectioning (3D) FLIM systems work in combination with LaVision BioTec's innovative TriM Scope technology which provides diffraction-limited 3D resolution of the fluorophores. |
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Specifications |
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| Image Intensifier Gate: down to 200 ps FWHM Repetition Rate: 110 Mhz Jitter: <20 ps Resolution Delay Unit: 25 ps Range Delay Unit: 0 to 20 ns Internal Delay: 6 ns System Dynamics: 12 bit Sensitivity: typical >100 counts/photoelectron Spatial Resolution: >20 line pairs/mm Spectral Range: 190 to 900 nm (depending on selection of intensifier tube) CCD Sensor: Sony 1340x1024 pixel Frame Rate: 10 Hz Dark Current: <0.1 e-/pixel*s (@ -12°C) Cooling: Two stage peltier cooling |
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| Components | |||
| LaVision HRI image intensifier, ps delay unit, LaVision Imager Intense CCD, advanced Pentium PC, Microsoft Windows, LaVision BioTec ImSpector software package |
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| Options | |||
| Image intensifier gate 100 or 50 ps, 16 bit dynamics, optics, microscopes, lasers, combination with LaVision BioTec's TriM Scope |
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