Micro Titer Plate Imaging UV Excitation High Resolution ImagingAll versions utilize LaVision BioTec‘s innovative white light excitation concept in combination with up to date CCD technology. Both provides unmatched flexibility. Just the change of standard filters adapts the instrument on almost any dye.
Two different fluorescence illumination concepts are designed to match the application.
Dark Field Fluorescence illumination
This technique delivers high signal to noise ratio and provides a sensitivity that is better than 0,06 fluorophores per µm2 – but the technique is limited to flat substrates.
EPI fluorescence illumination
EPI fluorescence is not limited to flat substrates as it excites and detects fluorescence by common objective lens from the top. Thus it is the perfect choice for microtiterplate imaging.
An EPI-fluorescence system has been developed to combine optical resolution of 5µm
per pixel with high numerical aperture and therefore brilliant sensitivity over spectral range from 395-750nm.
Fast motorized sample positioning allows overviews with full resolution up to 180x200mm2 within one image. FIPS software allows fully automated plate movement and reading of (e.g.) 96 wells within 30 seconds. Additionally, sub-grids and sub-areas can be user defined and evaluated automatically.
while reading a 384 well plate under
Cy3 illumination.
High aperture and long distance objective lens has been introduced into the optical setup. Mechanical stability of the assembly has been improved to suppress smearing effects of the image. With standard CCD, one image covers an area of 5.0x3.8mm2. Fast motorized sample positioning allows overviews with full resolution up to 40x50mm2 within one image. Dark field illumination of the sample gives brilliant images and excellent contrast.
Cy3- and Cy5 channels are merged
CCD Integration Time 150ms
Quartz Lenses in Excitation Light Path
UV transmitting light guide
Special Filters
The UV Kit option allows the user to excite native Fluorescence and UV dyes
