Endomicroscope - LaVision BioTec GmbH

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Endomicroscope

360° Panorama View Endomicroscope

In vivo imaging with sub-cellular resolution is limited to a depth of 1 mm due to the tissue’s optical density, scattering and absorption of the emitted fluorescence light. 2-photon endomicroscopy as a minimal invasive approach enlarges the imaging volume by at least one order of magnitude by going deeper into the tissue and recording 360° panorama images! Moreover, spiral panorama images of e.g. the striatum deep in the mouse brain can be recorded.
The flexible design enables the implementation into LaVision BioTec’s TriM Scope II but also in existing standard two-photon microscopes from other manufacturers.   


Benefits
  • Deepest in vivo imaging
  • 360° Panorama View imaging
  • Spiral Panorama Sets
  • Minimal invasive
  • Sub-cellular resolution
 




Principle of operation
The Endomicroscope optics consist of a standard laser-scanning objective lens and an additional rod lens in the tip. A prism at the tip deflects light by 90° and therefore allows side viewing. Furthermore, motorized rotation of the rod lens allows 360° panorama view dataset recording deep inside live tissue. The whole Endomicroscope can be moved along the microscope’s optical axis by a linear precision drive. This allows to change the depth within the tissue and recording of spiral panorama datasets.
 

Imaging Modes
The rotational side view endoscope allows different imaging modes.  
  
360° Panorama View:
At a selected depth within the tissue motorized rotation of the rod lens enables 360° panorama view 2D or 3D recordings.
 
 
3D Volume Acquisition:     
A 2D image is recorded by scanning the endoscope focus with the scanner technology. Motorized changing of the focus in front of the endoscope allows it to address consecutive z planes in order to record a 3D dataset.
 
360° Spiral Panorama View:
By consecutive z-movement of the whole endoscope in combination with rotation of the rod lens, 360° Spiral Panorama 2D or 3D datasets can be recorded.
 
 
 




Integration into LaVision BioTec’s TriM Scope II
The microendoscope is designed as an upgrade for LaVision BioTec’s TriM Scope II. Due to the modular design, the user can easily switch between standard and endoscope mode without re-alignment.
The Endoscope is combinable with LaVision BioTec’s
  • Resonant scanner
  • 4X Cloud Scanner
  • Cloud Scanner
  • Adaptive Optics Module
  • Spectral Detector
  • FLIM detector
 
LaVision BioTec’s TriM Scope II with installed Endomicroscope
 
 

Integration into further 2-photon microscopes
Alternatively, a mechanical kit allows the endoscope device to be mounted directly on the optical table enabling implementation into laser-scanning microscopes from other manufacturers.
 

Specifications
Magnification                                  3.8                              
Numerical aperture                         0.65
Working distance                            200 µm
Field of view                                    150 µm2
Tip diameter                                    2 mm
Tip length                                        6 or 18 mm
Excitation spectral range                800 – 1300 nm
Detection spectral range                420 – 800 nm
Side view angle                               90°
Anti-reflective coating on all surfaces
Bio-compatible stainless steel shell around the endoscope tip


 
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