TriM Scope II Scanner Options - LaVision BioTec GmbH

Advanced Microscopy
Go to content

Main menu:

Products > 2-Photon Microscope


TriM Scope II - Scanner Options

The TriM Scope II offers different combination of galvo and resonant scanners:

  • 2 galvanometric scanners with 2.5 frames/sec @ 512x512 px resolution in the unidirectional mode
  • Fast resonant scanner with > 30 frames/sec @ 512x512 px resolution
  • The alternative use of galvo or resonant scanner
  • Second independent galvanometric scanner additional to the existing galvo or resonant scanner

Beside the standard configuration with two galvo scanners for 3D imaging, line scanning and local photo-treatment, the user can add a resonant scanner to its system. With more than 30 frames/sec @ 512x512 pixel resolution, the resonant scanner allows fast imaging of e.g. calcium signals.
By using the resonant scanner in combination with a piezo driven z-stepper, fast 3D imaging of large volumes is possible. This enables e.g. the observation of signal forwarding in the 3D neuronal networks of living animals or cell movement trough the tissue.
For photo-treatment with a galvo scanner and fast imaging with a resonant scanner, a second independent galvo scanner can be added to the TriM Scope II. This configuration enables fast imaging with the resonant scanner during photo-activation, stimulation, or optogenetic modulation via the independent galvo scanner.


Scanfeatures
·         ROI-scanning (arbitrary speed and field dimension)
·         Off center fields, large scans, line averaging
·         Line scanning (high speed single line, polygon scanning)
·         Multipoint access, mixed scan modes
·         Photo-treatment of single and multiple ROIs, laser cutting
·         Fast resonant scanning
·         Fast 3D imaging with resonant scanner and piezo driven z-stepper
·         Independent 2nd galvo scanner for simultaneous imaging and treatment


Scan rates of the 2 axis galvanometric XY-scanner
Max. number of pixel: 
14500 x 14500 unidirectional,
1000 x 1000 bidirectional

Scan rates @ 250 x 250 pixel resolution in the unidirectional scan mode:
FOV 
[µm x µm], 20x Objective lens

max scanner frequency [Hz]

min frame time [ms]max frame rate [Hz]
500 x 500
800
397
2.5
400 x 400
1200
294
3.4
300 x 300
1600
241
4.1
200 x 200
2000
207
4.8
100 x 100
2400
190
5.2
50 x 50
2600
178
5.6

Scan rates @ 250 x 250 pixel resolution in the bidirectional scan mode:
FOV 
[µm x µm], 20x Objective lens

max scanner frequency [Hz]

min frame time [ms]max frame rate [Hz]
500 x 500
2000
129
7.7
400 x 400
2500
106
9.3
300 x 300
3000
8911.1
200 x 200
3500
78
12.8
100 x 100
4000
66
15.0
50 x 50
4000
66
15.0


 
Back to content | Back to main menu