TriM Scope II - Introduction
The TriM Scope II is a modular 2-photon microscope that can be adapted to a various number of applications: from developmental biology, cancer research and immunology to neuroscience. Thereby, different types of measurements from 3D timelapse experiments to fast calcium imaging are possible.read more >>
The TriM Scope II 2-Photon microscope can be combined with upright and inverted stands from different companies. Beside LaVision BioTec’s own upright intravital stand, also stands from Nikon, Olympus, or Zeiss can be integrated into the Trim Scope II. In addition, LaVision BioTec’s Intravital Stage can be used as a sample platform.read more >>
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The TriM Scope II offers different combination of galvo and resonant scanners. Beside the standard configuration with two galvo scanners for 3D imaging, line scanning, and local photo-treatment, the user can add a resonant scanner to its system. For advanced photo-treatment experiments, a second independent scanner can be integrated.
For many applications like calcium imaging, a high frame rate is essential to record all important events. In addition, to protect the sample and enable long time measurements, the photodamage of the sample has to be reduced. Both conditions can be achieved by using a device that splits one beam into multiple beams. This beam multiplexing is realized by LaVision BioTec’s 4X Cloud Scanner, Cloud Scanner, and the 64 Beam Option.read more >>
The fluorescence lifetime is a unique characteristic of a fluorophore. Because it varies with changes in the local environment (chemical and molecular) of the fluorophore, it can be used in Fluorescence Lifetime Imaging Microscopy (FLIM) to observe changes in the local environment that cannot be observed with standard fluorescence microscopy. LaVision BioTec’s FLIM detector now allows fast and high sensitive FLIM measurements.read more >>
intravital imaging the focus quality is often reduced when the excitation beam propagates through the tissue. The different parts of a tissue can have different refractive indexes that lead to tissue induced aberrations. With a deformable mirror these aberrations can be corrected by LaVision BioTec’s Adaptive Optics Module.read more >>
The specification list includes all information about laser sources, scanners, beam multiplexer, stands, stages, detectors, and measurement modes that can be realized with the TriM Scope II.
- Fast intravital imaging with multiplexed beams and resonant scanner- FRAP, photo-activation / -stimulation, uncaging, optogenetics, and line scanning- SHG and THG imaging with OPO and Ti:Sa laser- Integrated FLIM imaging with LaVision BioTec’s FLIM detector- Ultrafast 64 beam imaging with up to 100 frames/s @ 2560x2160 pixel
- Excitation of red dyes- Reduced phototoxicity and bleaching- High imaging depth with 1100 - 1600 nm @ 200 fs- Optimized SHG and THG excitation and detection
Hardware features of the TriMScope II- Combination of up to 2 Ti:Sa + OPO- Chirp compensation optional- Two internal EOMs, up to 8 EOM or AOM in total- Adjustment control with 4 Quadrant Diodes- Variable telescope, large diameter optics- Motorized tubelens- Various number of detectorsScanfeatures- ROI scanning- Line scanning (high speed single line, polygon scanning)- Mulitpoint access, mixed scan modes- Line averaging scans- Mosaic scans for large scan fieldsAdvanced Photo-treatment- Photo-treatment in single and multiple ROIs- Laser cutting- Local power adaption for imaging and treatment- Visible and UV lasers for photo-activation and photo-switching