UltraMicroscope II Specifications - LaVision BioTec GmbH

Advanced Microscopy
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UltraMicroscope II - Specifications


The Ultramicroscope Setup
The advanced Ultramicroscope configuration delivers superior imaging capabilities and user friendliness. Just choose camera, objective lens and laser light source to adapt the Ultramicroscope to your application.   

  • Newest sCMOS technology for high quality images   

  • Bidirectional sheet excitation allows a consistent illumination   

  • Low magnification lenses for macro imaging


Laser Sources

  • Laser module with up to 5 laser diodes  

  • White light super continuum lasers for maximum excitation flexibility




Sheet optics
Illumination
Uni- & bidirectional
Number of light sheets
3 – 6
Thickness
4 µm – 24 µm
         
Numerical   aperture
0.0135 – 0.135
Focus positioning
Dynamic
Refractive index matching
1.33 – 1.56
Zoom optics for 2x objective lens Zoom
Mono zoom
Zoom ratio
0.63x – 6.3x (1:10)
Detection optics
Objective lenses
2x
Numerical aperture
0.5
FOV diagonal (mm)
1.7 - 17.6
Total magnification (objective lens + zoom ratio)
1.26x – 12.6x
Working distance
4 mm, 6 mm, 10 mm
Refractive index matching
1.33 – 1.56
Chromatic detection
Eight filters
Chromatic correction
Dynamic 400 nm – 850 nm
Detector
Type
sCMOS
Pixel
2560 x 2160
Pixel size
6.5 µm x 6.5 µm
Maximum frame rate
100 fps @ full frame
Read noise
1 e-
Imaging chamber
Imaging solution
aqueous buffers and organic solvents
Sample travel range (X, Y, Z)
1 cm, 1 cm, 1 cm
Sample size
µm Range to cm Range
Light source
Laser module
Max. 5 Laser Lines , 50 mW - 100 mW per diode
Supercontinuum laser
Emission 460 nm – 800 nm, 1 mW/nm – 3 mW/nm
Dimensions
54 cm x 70 cm x 65 cm (W x H x D)
Weight
47 kg (w/o controller and laser)
 

Zoom factor and corresponding NA and resolution by pixel size:
zoom factor
magnification by zoom body and 2x objective lensNA detection path
optical resolution by NA
pixel size (Andor Neo) [µm]resolution [µm] by detector
0.63
1.26
0,07
4,36
5.16
10.32
0.8
1.60.07
3.39
48
1
20.11
2.76
3.256.5
1.25
2.50.14
2.18
2.65.2
1.6
3.20.19
1.6
24
2
40.23
1.33
1.623.24
2.5
50.295
1.02
1.32.6
3.2
6.40.38
0.8
1.012.02
4
80.475
0.64
0.81.6
5
100.495
0.62
0.651.3
6.3
12.6
0.495
0.62
0.511.02
 

 
Light sheet thickness as a function of the NA:
light sheet NAlight sheet thickness [µm]
0.01
12.4
0.05
9.5
0.07
7.2
0.1
6
0.148
4
   



 
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