Bidirectional Triple Light Sheet Microscopy (BTLSM)
Exciting the fluorescent sample perpendicular to the detection by a focused light sheet offers two major advantages: First, it allows 3D microscopy utilizing a wide field microscope. A camera-based wide field microscope delivers much higher frame rates than any laser scanning microscope. Second, the combination of wide field microscopy and sheet excitation reduces bleaching and photo toxicity significantly. LaVision BioTec’s UltraMicroscope II comes with up to six light sheets that excite the sample from different angles. This means the fluorescence excitation is most homogenous and artifacts like dark areas and stripes are minimized.